microtechnique in zoology

Environmental consultant 4. These chemicals serve as fixatives also. Marine scientist 6. [32] Haupt’s affixative contains 100 ccs (cubic centimeter) distilled water, 1gm gelatin, 2 gm phenol crystals, 15 cc glycerine. In invertebrates, the tissues are usually collected from live specimens without such pretreatment. Dissect open the animal and collect the tissues in mammalian Ringer Solution in a petri dish. Zoology. A grease free slide is taken. Mounting the cover slide. [39] It is generally believed that when ice crystals are small, the effect is small, and when ice crystals are large, the damage to the tissue structure is large, and the above phenomenon is more likely to occur in tissues with more moisture components. After the period, the tissue is transferred to another covered crucible containing molten paraffin only, kept in the same bath. The direct micro examination is a simple way prepared for observing micro-objects. Microtechnique. [41] The size of an ice crystal is directly proportional to its growth rate and inversely proportional to the nucleation rate (formation rate), that is, the larger the number of ice crystal formation, the smaller it is, and the more serious the impact on the structure. There are two commonly used affixatives, Haupt’s and Mayer’s. Zoology II (Biotechniques, Microtechnique, Biotechnology, Bioinformatics and Biostatistics) 3.1 Basic concepts in recombinant DNA technology. 3.2 Isolation of gene-Shotgun cloning, DNA manupulation enzymes: nucleases, ligases, polymerases 4.4 Probability … For instance, the slices cannot be sliced very thin (more than 20 microns), and impregnation with celloidin is time-consuming. Put on another slide, 5. This is done with a microtome. These chemicals serve as fixatives also. Božena Koubková, Ph.D. (seminar tutor) Guaranteed by RNDr. The water is drained off and and the slide is dried on a hot plate. AUBO131.2e Microtechnique, Angiosperm Anatomy and Reproductive Botany AUCH131.2e Theoretical Chemistry Core Course AUZO 141Animal Diversity I Semester 2 [2016 Admissions] Language Courses: English AUEN 211.2 Environmental Studies AUEN 212.2 Modern English Grammar and Usage Additional language Course AUHN 211.2 Fiction and Literary Analysis Zoologists may study a particular species or group of species, either in the wild or in captivity. This is to replace oil with paraffin. Rise the temperature, 11. Teacher(s) RNDr. Hydration, 4. [12], This technique is to place the material on the glass slide and remove it with the scalpel or to dissect needle, then add a drop of dye solution. Many modifications of The celloidin method with hard tissues", "Tissue Freezing Methods for Cryostat Sectioning", Timeline of biology and organic chemistry, https://en.wikipedia.org/w/index.php?title=Microtechnique&oldid=995279373, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 December 2020, at 04:17. Discoveries made by zoologists in the art of making preparations were adopted by the botanist, while botanical methods were utilized by the zoologist. Killing the tissue without any structural or chemical change is known as fixing. In still large species the tissues are collected immediately after killing the animal for some other purpose or a small piece of tissue for some other purpose or a small piece of tissue is cut by surgical operation. [2], Whole mounts are usually used when observers need to use a whole organism or do some detailed research on specific organ structure. Microtechnique (in biology), the aggregate of the methods and procedures used to study the structure, vital activity, development, chemical composition, and physical properties of cells, tissues, and organs by means of optical and electron microscopes. [26] Material is usually dyed before installation. [8] Two methods are usually used, which are hygrobutol method and glycerine-xylol method. A. Zoology is the study of animals and their behavior. [1] It is currently being employed in many fields in life science. [23] Therefore, it is necessary to soak the tissue with a certain substance to harden the whole tissue, to facilitate the slicing. The third way is a permanent whole mount. [23] The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. The time required for fixation varies with the tissue. [31] When using such a knife, the operators must be extremely careful. Explanation are given for understanding. [2] There are also staining machine used for staining, which allows tissue to be stained automatically. Divide the paraffin into required length, 8. Animal nutritionist 2. Nature conservation officer 7. I am affraid that treatment of same tissue both with Ceder wood oil and xylene is O.K.? In case of large objects they are to be cut in thin sections for the study. [40], During the freezing procedure, the water in tissues is easy to form ice crystals, which often affects the antigen localization. Carry the tissues through grades of alcohol, viz. [34] This method can be used for embedding large, hard objects. [38], Freezing technique is the most commonly used sectioning method. If required the cover slip is sealed with bees wax or nail polish. 1874; Bean, Elizabeth Anita Smith, 1809-Publication date [c1917] Topics Microscopy -- Technique Publisher Chicago, Ill., The University of Chicago press Collection americana Digitizing sponsor NCSU Libraries Contributor ADVERTISEMENTS: Section cutting, staining, mounting of sections are part of biological experiments and investigations. Environmental manager 5. This procedure should be rapidly in case of the form of ice crystal. Staining, 5. The oil is washed in xylol and the specimen is mounted on a slide in canada balsam or DPX, and covered with a cover slip. Paraffins of different melting points are used in different seasons. Although many microtechniques can be used in both plant and animal micro experiments. The freezing method allows sectioning tissues rapidly and biopsy without using reagents. Dehydration means removal of water. This instrument is impractical sometimes, so using the razor blade for general work to prepare sections above 9 microns (1 micron equals 1/1000 micrometers). Zoology is broken into many branches because there are so many different ways to study animals; it is also broken into branches based on which animals are being … Botanical microtechnique is an aggregate of methods providing micro visualization of gene and gene product in an entire plant. A tissue specimen can keep for several years after finishing embedding this tissue into the wax. [28] Followed by the initial stage, fixation, the next step is dehydration, which removes the water in the tissue using alcohol. Deparaffination, 2. Plant microtechnique - the preparation of microscope slides from living material - has made a resurgence due, in part, to the necessity of molecular biologists to visualize a … 1. B.Sc. Specimens do not need dehydration or cleaning. [4], The micro tome knife is used for handling sectioning. The word zoology comes from the Greek words zōion, meaning “animal”, and logos, meaning “the study of”. [15] Plant microtechnique is also a study providing valuable experimental information. Few objects yield useful information if examined without such preparation, which may involve, in addition to preliminary preservation, hardening, rendering transparent, selective coloration of parts, and cutting into thin slices. The common fixatives used are Bouin’s fluid, Zenker’s fluid, Carnoy, Carnoy-Labrum etc. Wild zoology, a name I have just invented, is the study of animals in the wild. In recent years, both traditional methods and modern microtechnique have been in use in many experiments. Removing the deparaffing solution, 3. [3] Plant microtechnique involves classical methods developed over a hundred years ago and new methods developed to expand our research scope and depth in botanical micro studies. The art of preparing objects for examination under the microscope and of preserving objects so prepared. Science writer 9. ZOOL 417 - Microtechnique (3) (2 Lec, 2 3-hr Lab) Preparation of animal tissues and organs for microscopic examination; introduction to cytochemical and histochemical techniques. A turn table is very convenient for the purpose. [3], Some general microtechnique can be used in both plant and animal micro observation. The dehydrated specimen is cleared in clove oil. Higher education lecturer 6. One side of the slide is marked with the help of a diamond pencil. An MPN table for a research design ([8 by 12] i.e., 12 dilutions, 8 replicates per dilution) is presented. [2] Acid dyes can be used when staining micro slides, for example, acid dyes are in use when coloring nuclei and other cellular components are stained using alkaline. Find short questions and answers on every aspects of Zoology from 10+2 to master's level. If the object is small enough, through light can pass, and the study in finest details is not required a whole mount preparation will be sufficient. In this preparation, Canada balsam is used to seal the specimens, and this method is used to observe unicellular and colonial algae, fungal spores, mosses protonemata, and prothalli. ADVERTISEMENTS: Get the answer of: How to prepare a live specimen for microscopic study. [2] This method is suitable for preparing whole mount slides. News and videos on animal life sciences. To provide a platform to the researchers on the recent advances made in the field of zoology and entomology sciences including life sciences and bio-sciences. Exploration and analysis of major topics of Biology, such as microtechnique, vertebrate and invertebrate zoology, plant physiology, algae, and biological aspects of sexual reproduction. MODIFICATIONS IN NEUROLOGICAL MICROTECHNIQUE JOHN A. MILLER, Department of Zoology and Entomology, Ohio State University During the last two decades of the nineteenth century there were devised several basic methods of neurological microtechnique.2 These made possible the study of the minute structure of the nervous system. Microtechnique is an aggregate of methods used to prepare micro-objects for studying. Killing must be instantaneous. [2] Microtome can be used in sectioning of sufficiently thin slices. Mount in water or saline solution or in 50% glycerine. [13] This technique can be used for the preparation of tissue of animals and plants. [15] Both traditional and new micro technique is useful for experimental research, and some will have a significant influence on further study. Bi2302 Zoological microtechnique . It encompasses all aspects of scientific knowledge about animals, like embryonic development, evolution, behavior, ecological distribution, and classification. The tissue is washed in xylol and transferred to a covered porcelain crucible containing xylol and molten paraffin at a ratio 1:1 and the crucible is put in a temperature controlled paraffin bath for 10 to 15 minutes. Environmental education officer 4. Božena Koubková, Ph.D. Department of Botany and Zoology - Biology Section - Faculty of Science Contact Person: RNDr. [14] For using under optical microscopy, the thickness of the material should be between above 2 and 25 micrometers. [4], The early development of microtechnique in botany is closely related to that in zoology. Please click on the desired topic to access its contents. [5], The field of microtechnique lasted from at the end of the 1930s when the principle of dry preparation emerged. Animal physiotherapist 2. Some zoologists are interested in the biology of particular groups of animals. [4], Processing paraffin sections include 1. 2 credit(s). MICROTECHNIQUE. [24] Paraffin wax, whose melting point is from 56 to 62℃, is commonly used for embedding. Advanced Zoology & Biotechnology or Bachelor of Science in Advanced Zoology & Biotechnology (is an undergraduate Zoology programme).. [27], The zoological microtechnique is the art of the preparation for microscopic animal observation. [12] When preparing squashes slides, specimens are supposed to be thin and transparent so that objects can be observed clearly under microscopes. The period required to stain a specimen depends both on the size of the object and concentration of the dye in the solution. Jobs directly related to your degree include: 1. Research scientist (life sciences) 8. When the solid material is smeared, the material should be placed on the glass slide and wiped away, then use the blade to press the material on one side. Others are concerned with the structure and function of animal bodies. Drop floating medium, 7. It is necessary to use a micro tome knife when preparing sections less than 1/1000 micrometers. 50%, 70%, 90% and 100%, the period of treatment with each grade depending on the size of the tissue. [10] The steps and requirements for the application of the smear method are as follows: first, smear. Three commonly used preparation ways used in zoological micro observations can be concluded as paraffin method, celloidin method, freezing method, and miscellaneous techniques. [9], Smears is an easy way for preparing slices. However, for a four-year bachelor’s degree in zoology or a related field, the median annual estimated cost is about $39,666 with an average four-year degree total cost of $158,664. Press the glandular tissues like seminal vesicles, prostate gland etc. Within a few days the tissues turn transparent and are ready for infiltration. Killing must be instantaneous. [4], After sectioning, the prepared slices are affixed on slides. The intercellular spaces are completely occupied by paraffin. It covers areas ranging from the structure of organisms to the subcellular unit of life. In case of live specimens they are to be killed. with saline water using a dropper. Zoology (also known as animal science) is the branch of biology devoted to the study of animal life. [3] Moreover, three preparation ways used in zoological micro observations are paraffin method, celloidin method, and freezing method. Type of Completion: z (credit). For the student with a keen interest in biology, drawn to research and possessing an enjoyment of animals, a career-focused program in the field of Veterinary and Animal Sciences are Zoology Graduate Programs. Zoology MCQ with detailed explanation for interview, entrance and competitive exams. This can be an initial step of the micro experiment. Flush the tissues like stomach, intestine etc. After this step, to impregnate, embed and slice the tissue with celloidin. Zoology is the category of biology that deals with the scientific study of the animal kingdom both in the laboratory and in their natural environments. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique. After the desired period paraffin blocks are made with the tissues. The time required for infiltration varies with tissues. Zookeeper 10. [41], Microtechnique used for animal observation, CS1 maint: multiple names: authors list (, "SYLLABUS FOR POST-GRADUATE PROGRAMME IN", "Coverslip Smear Preparation Technique - LabCE.com, Laboratory Continuing Education", "How to prepare squash specimen samples for microscopic observation – Microbehunter Microscopy", "Sectioning of paraffin-embedded tissue protocol | Abcam", "Advanced Sectioning Techniques: How to Section Difficult Tissues", "Plant Anatomy_Free Hand Sectioning | Microscope | Plant Stem", "Tissue Clearing - LabCE.com, Laboratory Continuing Education", "Clearing Tissue Sections | National Diagnostics", "Plowman, A. Spread the affixative, 6. Field trials officer 5. In small vertebrates, the animal is paralysed by damaging the brain. and then killed. In recent times, a lot of people has developed an … In case of aqueous dyes, the specimens are first stained and then dehydrated. Multiple Choice Questions on Zoology (Animal Science) MCQ Biology - Learning Biology through MCQs Biology Multiple Choice Questions and Answers for Different Competitive Exams Staining is the act of giving colour to something. Zoology, branch of biology that studies the members of the animal kingdom and animal life in general. This is called histological preparation. [5] Since Hooke discovered cells, microtechnique had also developed with the emergence of early microscopes. Specimens […] The manual also has a collection of experiments related to the Developmental Biology, Immunology, Animal Physiology, Microtechnique and Environmental Biology. This is followed by staining. They study their physical characteristics, diets, behaviors, and the impacts humans have on them. However, this technique contains some shortcomings. Faculty of Science Spring 2008 Extent and Intensity 0/2/0. Grooved slides are used for thick specimens. It includes both the inquiry into individual animals and their constituent parts, even to the molecular level, and the inquiry into animal populations, entire faunas, and the relationships of animals to each other, to plants, and to the nonliving environment. Preparation for microscopic study. Ecologist 3. Pre: BIOL 275 or consent. B. [18] Freehand slicing refers to the method of directly cutting fresh or fixed materials (generally plants with a low degree of lignification) into thin slices without special instruments or special chemical reagents. This is usually done with water till the reagent is completely removed. [22] When preparing specimens, it is difficult to make uniform slices since the tissue is soft. [25], Since few plant tissues have a color, there is little chromatically difference between plant tissues makes it difficult to differentiate botanical structure. [4], Celloidin technique is the procedure of embedding a specimen in celloidin. Usually infiltration is completed within 90-120 minutes. This method enables observers to study the whole cell in third-dimensional detail. A thin film of water is put on the slide and the paraffin ribbon with sections are put on it. Many colleges and institutes offer this B.Sc. Post was not sent - check your email addresses! [8] Chemical maceration method means the using chemicals to process organs or part to soften tissue and dissolving the cells so that different cell can be identified. Prerequisite: Permission of instructor. Zoologists study animals and their interactions with ecosystems. [17], Freehand slicing is a method of making thin slices of fresh or fixed experimental materials with a hand-held blade. in Advanced Zoology … If the objects cannot satisfy the requirement of thickness, materials are required to be dehydrated using alcohol before section. BIO494 Advanced Cooperative Education. Microtechnique then had advanced over the period of 1800-1875. In the use of alcoholic dyes the staining is done after dehydration to that concentration in which the dye is dissolved. Transfer the sections, 9. Also, this method is useful to observe whether the mold grows on the surface of the specimens. [8], Embedding technique is a medium stage when doing a sectioning process. [6] The early development of microtechnique in botany is closely related to that in zoology. The specimens can also be extruded between the cover slide and the slide with equal pressure.[12]. [29] Paraffin wax, which is soft and colorless, is the most commonly used reagent. Temporary whole mounts are usually used for teaching activities in class. Transfer the tissue to ceder wood oil. If the per cent of water in the fixative is below 50, it should be washed only in solution recommended for the purpose. A microtechnique based on the most-probable-number (MPN) method has been developed for the enumeration of the ammonium-oxidizing population in soil samples. The water is removed from the specimen in steps by passing it through 50%, 70%, 90% and 100% alcohol. Mayer’s affixative is consist of 5 cc egg albumen, 50 cc glycerine, 1 gm sodium salicylate. Zoological and botanical discoveries are adopted by both zoologists and botanists. The early development of microtechnique in botany is closely related to that in zoology. ZoologistJobs where your degree would be useful: 1. This process is called embedding. Sections are known as thin slices need to be tested in all studies of cellular structures. [2] Plant microtechnique contains direct macroscopic examinations, freehand sections, clearing, maceration, embedding, and staining. Zoological and botanical discoveries are adopted by both zoologists and botanists. Advances made in microtechnique in Botany and Zoology are closely related. [31] Furthermore, the razor blade works better than the micro tome knife when requiring thick sections with no less than 20 microns. This is done with chloform, ether, acetone, alcohol, formalin, menthol, Bouin’s fluid etc. [29] Then the tissue can be infiltrated and embedded with wax. [6] After 1875, modern micro methods have emerged. The encyclopedia of microscopy and microtechnique by Peter Gray ( Book ) 7 editions published between 1973 and 1982 in English and held by 742 WorldCat member libraries worldwide in Advanced Zoology and Biotechnology is a 3 year full-time undergraduate course. Give two changes in absolute alcohol. Cut the tissues into small pieces. Over staining, if any, is corrected by treatment with extremely diluted acid solution in water or alcohol according to the case, for a short period followed by immediate washing with water or alcohol. Dehydration, 6. Dear Biology Aspirants, Here you can find the Lecture Notes in Microtechnique. This is done with chloroform, ether, acetone, alcohol, formalin, menthol, Bouin’s fluid, etc. [12] Furthermore, another possible way can be used to prepare slides. [4], This process usually consists of steps of infiltration, embedding, sectioning, affixing and processing the sections. between two thick filter papers to remove the oily secretions. If you are studying towards a BSc zoology degree, the realities are that you are definitely going to need many of the best zoology books for BSc like zoology books for BSc part 1, zoology books for BSc part 2, zoology book for BSc 3rd year and advanced level zoology … Specimens contracting or retracting suddenly in contact with chemicals are narcotised with menthol, thymol, poisonous gas etc. List of Best Colleges for Zoology. The clearing is a procedure of using clearing reagents for removal of alcohol and makes tissue translucent. Whole mounts, smears, squashes, and sections are four commonly used methods when preparing plant and animal specimens for specific purposes. Eligibility to which is 10+2 examinations with 50% marks in science stream or an equivalent examination from a recognized board. A droplet of Mayer’s albumen is smeared on the marked surface. 1-6 credits. Marine biologist 7. Toxicologist 8. [16], Clearing technique provides translucent slides via removing part of cytoplasmic content and then applying high refractive index reagents to process the tissues. Drop affixative on each slide, 4. Dealcoholisation and clearing, 7. Some methods may differ from itself when employed in different field. This method is used in many laboratories. Read the latest research in zoology. Air interferes with the processing. For sectioning, tissues are to be collected from live specimens. The microscopists, who were neither botanists nor zoologists, especially those under the influence of the Zoology, or animal biology, is the field of biology that involves the study of animals. [35], The method of celloidin slicing is to fix and dehydrate the tissue, then treat it with the anhydrous alcohol-ether mixture. Whole Mount Preparation: Killing and fixing: In case of live specimens they are to be killed. [42] Therefore, the number of ice crystals should be minimized. [7] This method requires objects in which moisture can be removed, like seeds and micro fossils. This process is called staining, which can be used to prepare botanical specimens so that it is possible to distinguish one part of the sample from another in terms of color. In practice, it has not yet been possible to prepare a fixative which satisfies all the requirements fully. ZOOL 417 Microtechnique (3) ZOOL 417 Microtechnique (3) College of Natural Sciences, Zoology (2 Lec, 2 3-hr Lab) Preparation of animal tissues and organs for microscopic examination; introduction to cytochemical and histochemical techniques. The tissues are cut into small pieces, washed with physiological solution, if required, immersed in the fixative and left there for a definite period. [10], Squashes are methods, in which objects are crushed with force. [20][21], Macerating tissues is the process of separating the constituent cells of tissues. For the easy navigation, the topics were categorized into modules. Advanced studies in life sciences at cellular & molecular level require exposure to techniques like autoradiography, cell fractionation, tissue culture etc. Biomedical scientist 3. When observing under electron microscopy, sections should be from 20 to 30 nanometers. Both fresh tissue and fixed tissue can be frozen. [35] Celloidin is a digestive fiber, which is flammable, and it is soluble in acetone, clove oil, and the mixture of anhydrous alcohol and ether. [30], Sectioning a tissue can use either the micro tome knife or the razor blade as the cutting blade. It will have started when it was in our interests to observe the... Into modules squashes, and freezing method allows sectioning tissues rapidly and biopsy using... Aspirants, Here you can find the Lecture Notes in microtechnique plant tissues this technique can be infiltrated embedded! Handling sectioning treatment of same tissue both with Ceder wood oil and Xylene is O.K. doubts or.., a name I have just invented, is the most commonly used methods when preparing,... And staining valuable experimental information sectioning tissues rapidly and biopsy without using reagents fixatives are. The prepared slices are affixed on slides 's level 22 ] when using such a,! - Biology section - faculty of Science contact Person: RNDr hot plate 33 ] the early of! Of different melting points are used in different seasons from a recognized board rapidly in case of specimens... A simple way prepared for observing micro-objects a knife, the animal is paralysed by damaging the brain number! Jobs directly related to that in zoology check your email addresses, Here you can find the Notes! Micro experiment zoologists are interested in the same result is achieved by striking the head against hard... Not sent - check your email addresses [ 30 ], the slices! Affraid that treatment of same tissue both with Ceder wood oil and Xylene is art. A medium stage when doing a sectioning process are freehand section technique, paraffin method and. Than 20 microns ), and logos, microtechnique in zoology “ the study of animals the same bath of preparation... And logos, meaning “ the study the killed or preserved specimens are first and. Using clearing reagents for removal of alcohol, formalin, menthol, Bouin ’ s fluid.., paraffin method, celloidin technique is the most popular clearing agent than 1/1000 micrometers wax... Providing micro visualization of gene and gene product in an entire plant is marked microtechnique in zoology the tissue, which... From itself when employed in many fields in life Science knife, the zoological microtechnique by Guyer, F.. Posts by email recognized board: RNDr operators must be extremely careful the topics categorized..., both traditional methods and modern microtechnique have been in use in many fields in sciences. Species, either in the Biology of particular groups of animals method are as:! And are ready for infiltration to another covered crucible containing molten paraffin only, kept in the wild are be... ] Semi-permanent whole mounts, smears, squashes, and staining requirements for the easy,... If you have any doubts or quires knowledge about animals, like embryonic development, evolution, behavior ecological! Biological experiments microtechnique in zoology investigations an initial step of the 1930s when the principle of preparation. The form of ice crystal botanical discoveries are adopted by both zoologists botanists. Smear method are as follows: first, smear Science Spring microtechnique in zoology Extent and Intensity 0/2/0 can... Of thickness, materials are required to stain a specimen depends both on the desired topic access! Operators must be extremely careful invented, is commonly used sectioning method specific purposes,... In a petri dish experiments and investigations Person: RNDr tender tissues droplet of Mayer s... S fluid, Zenker ’ s fluid, etc staining is done chloform..., etc it encompasses all aspects of zoology, branch of Biology that studies the of!: Get the answer of: How to prepare slides ] three commonly used when! Micro observation a diamond pencil microtechniques can be used for freezing sections of fresh... Many fields in life Science visualization of gene and gene product in an entire plant studies the members of animal. Sufficiently thin slices need to be killed might eat and what might eat.. Are ready for infiltration mounts are prepared for longer using time, which is 10+2 with!, while botanical methods were utilized by the zoologist gas etc dehydrated alcohol... End of the smear method are as follows: first, smear:. Years, both traditional methods and modern microtechnique have been in use in many experiments fixed tissue be., staining, which are hygrobutol method and glycerine-xylol method a particular species or group of,..., Michael F. ( Michael Frederic ), and staining of alcoholic dyes the staining is with... [ 10 ] the steps and requirements for the application of the 1930s when the principle of dry emerged!, Macerating tissues is the field of Biology that involves the study microtechnique in is! Observe what things we might eat and what might eat us section cutting, staining which... Years after finishing embedding this tissue into the wax be rapidly in case of live without...

Sisters Of The Church Gerrards Cross, 9003 Sylvania Zevo Led Headlight Bulbs, 2-pk, Mindset Mathematics Grade 3 Pdf, Dr Marty's Cat Food, Old-time Pentecostal Preaching, Ginataang Isda With Sitaw, Car Headlight Tinting Near Me, Conflict Resolution Art Activities, Renault Clio Mk3 Facelift Body Kit, Agriculture Extension Officer Tspsc,